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<head>
  <title>Start the 2P microscope</title>
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<h1>Startup</h1>
<h2>Switch on the Laser</h2>
<ol>
  <li>switch on chiller (black, lower switch)</li>
  <li>wait unitl 21&deg; (15 minutes), switch on main (orange, upper
switch)</li>
  <li>turn key on</li>
  <li>wait until Warmup 100% (see small display 20 min) <br>
  </li>
  <li>start Spectra-Physics Mai Tai software or use Tools Maitai in the
scan software
(humidity error is
normal when the laser was off a long time)</li>
</ol>
<h2>Switch on the Microscope</h2>
<ol>
  <li>switch on the imic-controller-box (OK: only the lowest green LED
on the DSP)<br>
  </li>
  <li>switch on the galvo controller (OK; Galvos all LED off,
Galvocontroller all green LEDs on)<br>
  </li>
  <li>Start ScanMicroscope.vi</li>
  <li>missing init the imic-motors!<br>
  </li>
  <li>run Single <br>
  </li>
  <li>change only once to no (down) and run (takes contionous images) <br>
  </li>
  <li>open shutter: Tools Maitai - Shutter open<br>
  </li>
  <li>adjust LUT: IMAQ Imaq-scale</li>
  <li>adjust high voltage to ~800V: Edit PMT-gain</li>
  <li>adjust z to "176": iMic z</li>
</ol>
<h1>Shutdown</h1>
<ol>
  <li>switch off high Voltage: Edit: PMT-gain</li>
  <li>switch off laser: Tools Maitai</li>
  <li>switch off the Maitai Key (Mainswitch and chiller should stay on)</li>
  <li>switch off iMic, Galvos, PMT-supply...</li>
</ol>
<br>
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